Journal: Journal of Clinical Microbiology
Article Title: In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus
doi: 10.1128/JCM.02861-15
Figure Lengend Snippet: Bioluminescent imaging of the equine uterus at 5 days postinoculation with lux-labeled P. aeruginosa. Luminescence was detected following repeated washing of the endometrial surface of the uterine body, indicating that the bioluminescent biofilm-like material produced by lux-labeled P. aeruginosa was strongly adherent and difficult to remove from the endometrium.
Article Snippet: After final dilutions, 150 μl was transferred to a 96-well minimum biofilm eradication concentration (MBEC) microtiter plate (Innovotech, Inc., Edmonton, Alberta, Canada).
Techniques: Imaging, Labeling, Produced